Inglis, Douglas, Ph.D.
Research ScientistContact Information:
5403 - 1 Avenue South
PO Box 3000
Lethbridge, Alberta T1J 4B1
Telephone: 403-317-3355
Fax: 403-382-3156
Email: douglas.inglis@agr.gc.ca
Current Projects:
- Development and application of molecular methods for the rapid and accurate detection and quantification of human pathogenic Campylobacter and Arcobacter
- Development of prebiotics and/or probiotics for preventing the colonization of bovine gastro-intestinal tracts and subsequent release of human pathogenic Campylobacter and Arcobacter species on farm and during processing
- Elucidation of factors influencing colonization of and release of Campylobacter and Arcobacter species from the gastro-intestinal tract of bovine and their role in the epidemiology of campylobacteriosis and arcobacteriosis
- Management of enteric bacteria associated with cattle
- Management of pathogens and resistance to antimicrobial agents in foods of animal origin throughout the food continuum
- Pathogens and antibiotic use in beef: safety throughout the farm to food continuum
Research Publications:
The links below lead to abstracts with access to full text.2013:
- Reti, K.L., Thomas, M.C., Yanke, L.J., Selinger, L.B., and Inglis, G.D. (2013). "Effect of antimicrobial growth promoter administration on the intestinal microbiota of beef cattle.", Gut Pathogens, 5:8. doi : 10.1186/1757-4749-5-8
2012:
- Inglis, G.D., Thomas, M.C., Thomas, D.K., Kalmokoff, M.L., Brooks, S.P.J., and Selinger, L.B. (2012). "Molecular Methods to Measure Intestinal Bacteria: A Review.", Journal of AOAC International, 95(1), pp. 2-23. doi : 10.5740/jaoacint.SGE_Inglis
- Puhl, N.J., Uwiera, R.R.E., Yanke, L.J., Selinger, L.B., and Inglis, G.D. (2012). "Antibiotics conspicuously affect community profiles and richness, but not the density of bacterial cells associated with mucosa in the large and small intestines of mice.", Anaerobe, 18(1), pp. 67-75. doi : 10.1016/j.anaerobe.2011.12.007
- Thomas, M.C., Selinger, L.B., and Inglis, G.D. (2012). "Seasonal Diversity of Planktonic Protists in Southwestern Alberta Rivers over a One Year Period Revealed by T-RFLP and 18S rRNA Gene Libraries.", Applied and Environmental Microbiology, 78(16), pp. 5653-5660. doi : 10.1128/AEM.00237-12
2011:
- Costa, E., Uwiera, R.R.E., Kastelic, J.P., Selinger, L.B., and Inglis, G.D. (2011). "Non-therapeutic administration of a model antimicrobial growth promoter modulates intestinal immune responses.", Gut Pathogens, 3:14. doi : 10.1186/1757-4749-3-14
- Inglis, G.D., Boras, V.F., and Houde, A. (2011). "Enteric Campylobacteria and RNA viruses associated with healthy and diarrheic humans in the Chinook Health Region of Southwestern Alberta, Canada.", Journal of Clinical Microbiology, 49(1), pp. 209-219. doi : 10.1128/JCM.01220-10
- Inglis, G.D., Yanke, L.J., and Selinger, L.B. (2011). "Cutinolytic esterase activity of bacteria isolated from mixed-plant compost and characterization of a cutinase gene from Pseudomonas pseudoalcaligenes.", Canadian Journal of Microbiology / Revue canadienne de microbiologie, 57(11), pp. 902-913. doi : 10.1139/W11-083
- Kalischuk, L.D. and Inglis, G.D. (2011). "Comparative genotypic and pathogenic examination of Campylobacter concisus isolates from diarrheic and non-diarrheic humans.", BMC Microbiology, 11:53. doi : 10.1186/1471-2180-11-53
- Thomas, M.C., Thomas, D.K., Selinger, L.B., and Inglis, G.D. (2011). "Spyder, a new method for in silico design and assessment of 16S rDNA primers for molecular microbial ecology.", FEMS Microbiology Letters, 320(2), pp. 152-159. doi : 10.1111/j.1574-6968.2011.02302.x
- Xu, S., Inglis, G.D., Reuter, T.R., Clark, G.O., Belosevic, M., Leonard, J.J., and McAllister, T.A. (2011). "Biodegradation of specified risk material and characterization of actinobacterial communities in laboratory-scale composters.", Biodegradation, 22(5), pp. 1029-1043. doi : 10.1007/s10532-011-9461-5
2010:
- Alexander, T.W., Inglis, G.D., Yanke, L.J., Topp, E., Read, R.R., Reuter, T.R., and McAllister, T.A. (2010). "Farm-to-fork characterization of Escherichia coli associated with feedlot cattle with a known history of antimicrobial use.", International Journal of Food Microbiology, 137(1), pp. 40-48. doi : 10.1016/j.ijfoodmicro.2009.11.008
- Inglis, G.D., Kastelic, J.P., and Uwiera, R.R.E. (2010). "Catheterization of intestinal loops in ruminants does not adversely affect loop function.", Comparative Medicine, 60(6), pp. 469-478.
- Inglis, G.D., McAllister, T.A., Larney, F.J., and Topp, E. (2010). "Prolonged survival of Campylobacter species in bovine manure compost.", Applied and Environmental Microbiology, 76(4), pp. 1110-1119. doi : 10.1128/AEM.01902-09
- Kalischuk, L.D., Leggett, F.L., and Inglis, G.D. (2010). "Campylobacter jejuni induces transcytosis of commensal bacteria across the intestinal epithelium through M-like cells.", Gut Pathogens, 2:14. doi : 10.1186/1757-4749-2-14
- Reuter, T.R., Xu, W., Alexander, T.W., Gilroyed, B.H., Inglis, G.D., Larney, F.J., Stanford, K.I.M., and McAllister, T.A. (2010). "Biocontained carcass composting for control of infectious disease outbreak in livestock.", Journal of Visualized Experiments (JoVE), 39(Article No. e1946). doi : 10.3791/1946
2009:
- Costa, E., Puhl, N.J., Selinger, L.B., and Inglis, G.D. (2009). "Characterization of mucosa-associated bacterial communities of the mouse intestine by terminal restriction fragment length polymorphism: Utility of sampling strategies and methods to reduce single-stranded DNA artifacts.", Journal of Microbiological Methods, 78(2), pp. 175-180. doi : 10.1016/j.mimet.2009.05.011
- Kalischuk, L.D., Buret, A.G., Inglis, G.D. (2009). "Campylobacter jejuni induces transcellular translocation of commensal bacteria via lipid rafts.", Gut Pathogens, 1:2. doi : 10.1186/1757-4749-1-2
- Puhl, A.A., Selinger, L.B., McAllister, T.A., and Inglis, G.D. (2009). "Actinomadura keratinilytica sp. nov., a keratin-degrading actinobacterium isolated from bovine manure compost.", International Journal of Systematic and Evolutionary Microbiology (IJSEM), 59(4), pp. 828-834. doi : 10.1099/ijs.0.003640-0
- Uwiera, R.R.E., Kastelic, J.P., and Inglis, G.D. (2009). "Catheterization of intestinal loops in ruminants.", Journal of Visualized Experiments (JoVE), 28(Article No. e1301). doi : 10.3791/1301
- Xu, W., Reuter, T.R., Inglis, G.D., Larney, F.J., Alexander, T.W., Guan, J., Stanford, K.I.M., Xu, Y., and McAllister, T.A. (2009). "A biosecure composting system for disposal of cattle carcasses and manure following infectious disease outbreak.", Journal of Environmental Quality, 38(2), pp. 437-450. doi : 10.2134/jeq2008.0168
Other Science Related Publications:
2012:
- Inglis, G.D. and Uwiera, R.R.E. (2012). "Development of biorationale-based alternatives to antimicrobial growth promoters.", Alberta Livestock and Meat Agency, Edmonton, AB, Canada, 2012. (Presentation)
- Inglis, G.D. and Uwiera, R.R.E. (2012). "Enhancing intestinal health in Alberta: a system-based, multidisciplinary, and integrated approach.", Alberta Livestock and Meat Agency Ltd., Edmonton, AB, Canada, January 2012. (Presentation)
- Inglis, G.D., Enkerli, J., and Goettel, M.S. (2012). "Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales.", in Lacey, L. (ed.) - A Manual of Techniques in Insect Pathology. 2nd Edition, Academic Press, Chapter 7.
2011:
- Brooks, S.P.J., Green-Johnson, J.M., Inglis, G.D., Uwiera, R.R.E., and Kalmokoff, M.L. (2011). "Gut microbiology – a relatively unexplored domain.", in Moo-Young, M. et al. (eds.) - Comprehensive Biotechnology, 2nd Edition. Vol. 4, Pergamon Press, Oxford, UK & New York, USA, pp. 575-590.
- Costa, E., Uwiera, R.R.E., Kastelic, J.P., Selinger, L.B., and Inglis, G.D. (2011). "Non-therapeutic administration of chlortetracycline modulates immune responses to Citrobacter rodentium in mice.", Plant Bioactives Research in Canada. Canada-United Kingdom Gut and Health Workshop, AAFC-FRDC / AAC-CRDA, Saint-Hyacinthe, QC, Canada, February 24-25, 2011.
- Reti, K.L., Thomas, M.C., Costa, E., Yanke, L.J., Selinger, L.B., and Inglis, G.D. (2011). "Administration of antimicrobial growth promoters does not alter the intestinal microbiota of beef cattle.", Plant Bioactives Research in Canada. Canada-United Kingdom Gut and Health Workshop, AAFC-FRDC / AAC-CRDA, Saint-Hyacinthe, QC, Canada, February 24-25, 2011. (Poster)
2010:
- Houde, A., Poitras, E., Leblanc, D., Ward, P., Inglis, G.D., Boras, V.F., Loisy-Hamon, F., and Lebeau, B. (2010). "Aichi virus in Alberta, Canada: a one year examination of human diarrheic stool samples by RT-PCR.", in Méndez-Vilas A. (ed.) - Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Vol. 1, Nova Science Publishers Inc, pp. 767-772.
2009:
- Alexander, T.W., Inglis, G.D., Yanke, L.J., Topp, E., and McAllister, T.A. (2009). "Characterization of antimicrobial-resistant Escherichia coli from samples collected throughout processing of feedlot cattle at a commercial abattoir.", ADSA-CSAS-ASAS 2009 Joint Annual Meeting, Montréal Convention Center, Montréal, QC, Canada, July 12-16, 2009. (Poster)
- Inglis, G.D. and Sikorowski, P.P. (2009). "Entomopathogens and Insect Rearing.", in Schneider, J.C. (ed.) - Principles and Procedures for Rearing High Quality Insect, Mississippi State University, USA, Chapter 9.
- Inglis, G.D. and Sikorowski, P.P. (2009). "Microbial Contamination and Insect Rearing.", in Schneider, J.C. (ed.) - Principles and Procedures for Rearing High Quality Insect, Mississippi State University, USA, Chapter 8.