Hegedus, Dwayne, Ph.D.
Plant Protection and Molecular BiologyContact Information:
107 Science Place
Saskatoon, Saskatchewan S7N 0X2
Telephone: 306-956-7667
Fax: 306-956-7247
Areas of Expertise:
- Disease and insect resistance
- Plant, fungal and insect molecular biology and biochemistry
- Recombinant protein expression
Current Projects:
- Platforms for the expression of recombinant proteins in seeds. The focus is on developing systems to reduce endogenous seed storage protein accumulation and studying the biology of seed protein trafficking and comparmentalization.
- Insect genomics and proteomics platforms leading to the identification of novel targets and strategies for insect resistance. The focus is on insect digestive biochemistry.
- Sclerotinia sclerotiorum-Brassica napus interactions and identification of genes/markers for stem rot resistance in canola.
Affiliations:
- Adjunct Professor, Department of Food and Bio-Product Sciences, College of Agriculture, University of Saskatchewan, Saskatoon, SK, Canada
Research Publications:
The links below lead to abstracts with access to full text.2013:
- Bashi, Z.D., Rimmer, S.R., Khachatourians, G.G., and Hegedus, D.D. (2013). "Brassica napus polygalacturonase inhibitor proteins inhibit Sclerotinia sclerotiorum polygalacturonase enzymatic and necrotizing activities and delay symptoms in transgenic plants.", Canadian Journal of Microbiology / Revue canadienne de microbiologie, 59(2), pp. 79-86. doi : 10.1139/cjm-2012-0352
- Toprak, U., Baldwin, D., Erlandson, M.A., Gillott, C., Harris, S., and Hegedus, D.D. (2013). "In vitro and in vivo application of RNA interference for targeting genes involved in peritrophic matrix synthesis in a lepidopteran system.", Insect Science, 20(1), pp. 92-100. doi : 10.1111/j.1744-7917.2012.01562.x
- Withana-Gamage, T.S., Hegedus, D.D., Qiu, X., Yu, P., May, T., Lydiate, D.J., and Wanasundara, P.K.J.P.D. (2013). "Characterization of Arabidopsis thaliana lines with altered seed storage protein profiles using synchrotron-powered FT-IR spectromicroscopy.", Journal of Agricultural and Food Chemistry, 61(4), pp. 901-912. doi : 10.1021/jf304328n
2012:
- Bashi, Z.D., Rimmer, S.R., Khachatourians, G.G., and Hegedus, D.D. (2012). "Factors governing the regulation of Sclerotinia sclerotiorum cutinase A and polygalacturonase 1 during different stages of infection.", Canadian Journal of Microbiology / Revue canadienne de microbiologie, 58(5), pp. 605-616. doi : 10.1139/W2012-031
- Gruber, M.Y., Wu, L., Links, M.G., Gjetvaj, B., Durkin, J.M.H., Lewis, C.T., Sharpe, A.G., Lydiate, D.J., and Hegedus, D.D. (2012). "Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding.", Genome / Génome, 55(2), pp. 118-133. doi : 10.1139/G11-083
- Toprak, U., Harris, S., Baldwin, D., Theilmann, D.A., Gillott, C., Hegedus, D.D., and Erlandson, M.A. (2012). "The role of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: Selective degradation of host peritrophic matrix proteins.", Journal of General Virology, 93(4), pp. 744-753. doi : 10.1099/vir.0.038117-0
- Wei, S., Gruber, M.Y., Yu, B., Gao, M.-J., Khachatourians, G.G., Hegedus, D.D., Parkin, I.A.P., and Hannoufa, A. (2012). "Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network.", BMC Plant Biology, 12:169. doi : 10.1186/1471-2229-12-169
- Yu, B., Gruber, M.Y., Khachatourians, G.G., Zhou, R., Epp, D.J., Hegedus, D.D., Parkin, I.A.P., Welsch, R., and Hannoufa, A. (2012). "Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus.", Journal of Experimental Botany, 63(14), pp. 5189-5202. doi : 10.1093/jxb/ers179
2011:
- Gao, M.-J., Li, X.L., Lui, H., Gropp, G.M., Lydiate, D.J., Wei, S., and Hegedus, D.D. (2011). "ASIL1 is required for proper timing of seed filling in Arabidopsis.", Plant Signaling & Behavior, 6(12), pp. 1886-1888. doi : 10.4161/psb.6.12.18709
- Li, X.L., Gruber, M.Y., Hegedus, D.D., Lydiate, D.J., and Gao, M.-J. (2011). "Effects of a Coumarin Derivative, 4-Methylumbelliferone, on Seed Germination and Seedling Establishment in Arabidopsis.", Journal of Chemical Ecology, 37(8), pp. 880-890. doi : 10.1007/s10886-011-9987-3
- Terenius, O., Papanicolaou, A., Garbutt, J.S., Eleftherianos, I., Huvenne, H., Kanginakudru, S., Albrechtsen, M., An, C., Aymeric, J.-L., Barthel, A., Bebas, P., Bitra, K., Bravo, A., Chevalier, F., Collinge, D.P., Crava, C.M., de Maagd, R.A., Duvic, B., Erlandson, M.A., Faye, I., Felföldi, G., Fujiwara, H., Futahashi, R., Gandhe, A.S., Gatehouse, H.S., Gatehouse, L.N., Giebultowicz, J.M., Gómez, I., Grimmelikhuijzen, C.J.P., Groot, A.T., Hauser, F., Heckel, D.G., Hegedus, D.D., Hrycaj, S., Huang, L., Hull, J.J., Iatrou, K., Iga, M., Kanost, M.R., Kotwica, J., Li, C., Li, J., Liu, J., Lundmark, M., Matsumoto, S., Meyering-Vos, M., Millichap, P.J., Monteiro, A., Mrinal, N., Niimi, T., Nowara, D., Ohnishi, A., Oostra, V., Ozaki, K., Papakonstantinou, M., Popadic, A., Rajam, M.V., Saenko, S., Simpson, A.J., Soberón, M., Strand, M.R., Tomita, S., Toprak, U., Wang, P., Wee, C.W., Whyard, S., Zhang, W., Nagaraju, J., Gordon, R.H.J., Herrerop, S., Gordon, K., Smagghe, G., and Swevers, L. (2011). "RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design.", Journal of Insect Physiology, 57(2), pp. 231-245. doi : 10.1016/j.jinsphys.2010.11.006
- Withana-Gamage, T.S., Hegedus, D.D., Qiu, X., and Wanasundara, P.K.J.P.D. (2011). "In Silico Homology Modeling To Predict Functional Properties of Cruciferin.", Journal of Agricultural and Food Chemistry, 59(24), pp. 12925-12938. doi : 10.1021/jf201979a
2010:
- Bashi, Z.D., Hegedus, D.D., Buchwaldt, L., and Rimmer, S.R., Borhan, M.H. (2010). "Expression and regulation of Sclerotinia sclerotiorum necrosis and ethylene inducing peptides (NEPs).", Molecular Plant Pathology, 11(1), pp. 43-53. doi : 10.1111/j.1364-3703.2009.00571.x
- Bashi, Z.D., Khachatourians, G.G., and Hegedus, D.D. (2010). "Isolation of fungal homokaryotic lines from heterokaryotic transformants by sonic disruption of mycelia.", BioTechniques, 48(1), pp. 41-46. doi : 10.2144/000113243
- Erlandson, M.A., Hegedus, D.D., Baldwin, D., and Toprak, U. (2010). "Characterization of the Mamestra configurata (Lepidoptera: Noctuidae) larval midgut protease complement and adaptation to feeding on artificial diet, Brassica species and protease inhibitor.", Archives of Insect Biochemistry and Physiology, 75(2), pp. 70-91. doi : 10.1002/arch.20381
- Toprak, U., Baldwin, D., Erlandson, M.A., Gillott, C., Harris, S., and Hegedus, D.D. (2010). "Expression patterns of genes encoding proteins with peritrophin A domains and protein localization in Mamestra configurata.", Journal of Insect Physiology, 56(12), pp. 1711-1720. doi : 10.1016/j.jinsphys.2010.06.016
- Toprak, U., Baldwin, D., Erlandson, M.A., Gillott, C., and Hegedus, D.D. (2010). "Insect intestinal mucins and serine proteases associated with the peritrophic matrix from feeding, starved and moulting Mamestra configurata larvae.", Insect Molecular Biology, 19(2), pp. 163-175. doi : 10.1111/j.1365-2583.2009.00966.x
- Wei, S., Yu, B., Gruber, M.Y., Khachatourians, G.G., Hegedus, D.D., and Hannoufa, A. (2010). "Enhanced seed carotenoid levels and branching in transgenic Brassica napus expressing the Arabidopsis miR156b gene.", Journal of Agricultural and Food Chemistry, 58(17), pp. 9572-9578. doi : 10.1021/jf102635f
- Yu, B., Gruber, M.Y., Khachatourians, G.G., Hegedus, D.D., and Hannoufa, A. (2010). "Gene expression profiling of developing Brassica napus seed in relation to changes in major storage compounds.", Plant Science, 178(4), pp. 381-389. doi : 10.1016/j.plantsci.2010.02.007
2009:
- Gao, M.-J., Lydiate, D.J., Li, X.L., Lui, H., Gjetvaj, B., Hegedus, D.D., and Rozwadowski, K. (2009). "Repression of Seed Maturation Genes by a Trihelix Transcriptional Repressor in Arabidopsis Seedlings.", Plant Cell, 21(1), pp. 54-71. doi : 10.1105/tpc.108.061309
- Gruber, M.Y., Xu, N., Grenkow, L.F., Onyilagha, J.C., Soroka, J.J., Westcott, N.D., and Hegedus, D.D. (2009). "Responses of the crucifer flea beetle to brassica volatiles in an olfactometer.", Environmental Entomology, 38(5), pp. 1467-1479. doi : 10.1603/022.038.0515
- Hegedus, D.D., Erlandson, M.A., Gillott, C., and Toprak, U. (2009). "New Insights into Peritrophic Matrix Synthesis, Architecture, and Function.", Annual Review of Entomology, 54, pp. 285-302. doi : 10.1146/annurev.ento.54.110807.090559
- Xu, J., Baldwin, D., Kindrachuk, C., and Hegedus, D.D. (2009). "Comparative EST analysis of a Zoophthora radicans isolate derived from Pieris brassicae and an isogenic strain adapted to Plutella xylostella.", Microbiology, 155(1), pp. 174-185. doi : 10.1099/mic.0.022103-0
- Zhao, J.W., Buchwaldt, L., Rimmer, S.R., Brkic, M., Bekkaoui, D.R., and Hegedus, D.D. (2009). "Differential expression of duplicated peroxidase genes in the allotetraploid Brassica napus.", Plant Physiology and Biochemistry, 47(7), pp. 653-656. doi : 10.1016/j.plaphy.2009.03.002
- Zhao, J.W., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., and Hegedus, D.D. (2009). "Patterns of differential gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum.", Molecular Plant Pathology, 10(5), pp. 635-649. doi : 10.1111/j.1364-3703.2009.00558.x
Other Science Related Publications:
2012:
- Bahar, M.H., Soroka, J.J., Hegedus, D.D., and Dosdall, L.M. (2012). "Thermal tolerance and HSP70 gene expression in DBM and Diadegma insulare.", Entomological Society of Canada and the Entomological Society of Alberta 2012 Joint Annual Meeting, Coast Edmonton Plaza Hotel, Edmonton, AB, Canada, November 4-7, 2012, #7. (Abstract)
2011:
- Buchwaldt, L., Lydiate, D.J., Yu, F.Q., Hegedus, D.D., and Rimmer, S.R. (2011). "QTLs contributing to sclerotinia stem rot resistance in Brassica napus Zhongyou 821.", 13th International Rapeseed Congress, Prague, Czech Republic, June 5-9, 2011.
- Fu, F., Harrington, M., Hegedus, D.D., Bekkaoui, D.R., Parkin, I.A.P., and Buchwaldt, L. (2011). "Co-location of defence genes from expression studies with QTLs conferring sclerotinia resitance in Brassica napus.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011. (Workshop)
- Gyawali, S., Buchwaldt, L., Harrington, M., Parkin, I.A.P., Bekkaoui, D.R., Horner, K., Higgins, E.E., and Hegedus, D.D. (2011). "Association mapping of stem rot resistance in a world collection of Brassica napus.", 2011 Joint Annual Meeting of the American Phytopathology Society (APS) & International Association for the Plant Protection Sciences (IPPC), Honolulu, HI, USA, August 6-10, 2011.
- Hannoufa, A., Wei, S., Gruber, M.Y., Khachatourians, G.G., Wang, Y., Hegedus, D.D., and Parkin, I.A.P. (2011). "MicroRNA156: A new player in regulating carotenoid accumulation in plants.", Canadian Society of Plant Physiologists Eastern Regional Meeting & Plant Development Workshop / Congrès de la Société Canadienne de Physiologie Végétale & Congrès de Développement Végétale (Congrès Régional de l’Est), Carleton University, Ottawa, ON, Canada, December 2-3, 2011, SB-1, pp. 17.
- Kutcher, H.R., Dokken-Bouchard, F.L., Turkington, T.K., Fernando, W.G.D., Boyetchko, S.M., Buchwaldt, L., Hegedus, D.D., and Parkin, I.A.P. (2011). "Managing sclerotinia stem rot in canola.", Canola Research Summit meeting, Winnipeg, MB, Canada, April 12-13, 2011.
- Sanjeewa, W.G.T., Hegedus, D.D., Yu, P., Qiu, X., McIntosh, T.C., May, T.W., and Wanasundara, P.K.J.P.D. (2011). "Influence of seed storage protein composition on structural features of major crucifer proteins: Protein secondary structure study of Arabiopsis thaliana.", 102nd Annual Meeting of the American Oil Chemists' Society (AOCS) & Expo, Duke Energy Center, Cincinnati, OH, USA, May 1-4, 2011.
2009:
- Zhao, J., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., and Hegedus, D.D. (2009). "Patterns of gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum.", 14th International Sclerotinia Workshop, Wilmington, NC, USA, May 31-June 4, 2009. (Workshop)
- Zhao, J., Buchwaldt, L., Rimmer, S.R., Sharpe, A.G., McGregor, L., Bekkaoui, D.R., and Hegedus, D.D. (2009). "Patterns of gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum.", 7th Canadian Plant Genomics Workshop, Sheraton Cavalier Hotel, Saskatoon, SK, Canada, August 24-27, 2009. (Workshop)