Gruber, Margie, Ph.D.
Chercheuse scientifiqueCoordonnées :
107 Science Place
Saskatoon, (Saskatchewan) S7N 0X2
Téléphone : 306-956-7263
Télécopieur : 306-956-7247
Courriel : margie.gruber@agr.gc.ca
Domaines d'expertise :
- génétique moléculaire
- métabolisme secondaire des plantes
- résistance aux insectes grâce à l'antixénose
- qualité des fourrages
Projets en cours :
- Création de ressources en génétique moléculaire pour la voie de la biosynthèse des flavonoïdes et des tanins concentrés pour améliorer la qualité des fourrages et des semences, la durabilité des cultures et de l'environnement et la valeur nutraceutique et industrielle des cultures
Publications de recherche :
Les liens ci-dessous mènent aux résumés avec accès au texte intégral.2012 :
- Bai, Y, Yang, Q., Kang, J., Sun, Y., Gruber, M.Y., et Chao, Y. (2012). « Isolation and functional characterization of a Medicago sativa L. gene, MsLEA3-1. », Molecular Biology Reports, 39(3), p. 2883-2892. doi : 10.1007/s11033-011-1048-z
- Canam, T., Li, X., Holowachuk, J., Yu, M., Xia, J., Mandal, R., Krishnamurthy, R., Bouatra, S., Sinelnikov, I., Yu, B., Grenkow, L.F., Wishart, D.S., Steppuhn, H., Falk, K.C., Dumonceaux, T.J., et Gruber, M.Y. (2012). « Differential metabolite profiles and salinity tolerance between two genetically related brown-seeded and yellow-seeded Brassica carinata lines. », Plant Science, 198(January 2013), p. 17-26. doi : 10.1016/j.plantsci.2012.09.011
- Chen, T., Yang, Q., Gruber, M.Y., Kang, J., Sun, Y., Ding, W., Zhang, T., et Zhang, X. (2012). « Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity. », Molecular Biology Reports, 39(5), p. 6067-6075. doi : 10.1007/s11033-011-1421-y
- Chen, T., Yang, Q., Zhang, X., Ding, W., et Gruber, M.Y. (2012). « An alfalfa (Medicago sativa L.) ethylene response factor gene, MsERF11, enhances salt tolerance in transgenic Arabidopsis. », Plant Cell Reports, 31(9), p. 1737-1746. doi : 10.1007/s00299-012-1287-z
- Gruber, M.Y., Wu, L., Links, M.G., Gjetvaj, B., Durkin, J.M.H., Lewis, C.T., Sharpe, A.G., Lydiate, D.J., et Hegedus, D.D. (2012). « Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding. », Genome / Génome, 55(2), p. 118-133. doi : 10.1139/G11-083
- Hossain, Z., Amyot, L., McGarvey, B.D., Gruber, M.Y., Jung, J., et Hannoufa, A. (2012). « The Translation Elongation Factor eEF-1Bß1 Is Involved in Cell Wall Biosynthesis and Plant Development in Arabidopsis thaliana. », PLoS ONE, 7(1, Article No. e30425). doi : 10.1371/journal.pone.0030425
- Jonker, A., Gruber, M.Y., Wang, Y., Coulman, B.E., McKinnon, J.J., Christensen, D.A., et Yu, P. (2012). « Foam stability of leaves from anthocyanidinaccumulating Lc-alfalfa and relation to molecular structures detected by fourier-transformed infraredvibration spectroscopy. », Grass and Forage Science, 67(3), p. 369-381. doi : 10.1111/j.1365-2494.2012.00853.x
- Jonker, A., Gruber, M.Y., Wang, Y., Narvaez, N., Coulman, B.E., McKinnon, J.J., Christensen, D.A., Azarfar, A., et Yu, P. (2012). « Fermentation, degradation and microbial nitrogen partitioning for three forage colour phenotypes within anthocyanidin-accumulating Lc-alfalfa progeny. », Journal of the Science of Food and Agriculture, 92(11), p. 2265-2273. doi : 10.1002/jsfa.5619
- Li, Y., Sun, Y., Yang, Q., Kang, J., Zhang, T., Gruber, M.Y., et Fang, F. (2012). « Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP. », Molecular Biology Reports, 39(8), p. 8559-8569. doi : 10.1007/s11033-012-1712-y
- Mahmoudi, H., Baâtour, Olfa, Huang, A.J., Nasri, N., Salah, I.B., Tarchoune, I., Zaghdoudi, M., Gruber, M.Y., Lachaâl, M., Ouerghi, Z., et Hannoufa, A. (2012). « Verte and Romaine lettuce varieties (Lactuca sativa) show differential responses to high NaCl concentrations. », Journal of Plant Nutrition and Soil Science, 175(4), p. 641-648. doi : 10.1002/jpln.201100217
- Mahmoudi, H., Ben Nasri, A.M., Baâtour, Olfa, Huang, A.J., Tarchoune, I., Nasri, N., Rym, K., Gruber, M.Y., Lachaâl, M., Hannoufa, A., et Ouerghi, Z. (2012). « Differential response to elevated NaCl by antioxidant enzymes and gene transcripts in two contrasting lettuce genotypes. », Australian Journal of Crop Science, 6(4), p. 632-640.
- Mupondwa, E.K., Li, X., Tabil, L.G., Phani, A., Sokhansanj, S., Stumborg, M., Gruber, M.Y., et Laberge, S. (2012). « Technoeconomic analysis of wheat straw densification in the Canadian Prairie Province of Manitoba. », Bioresource Technology, 110, p. 355-363. doi : 10.1016/j.biortech.2012.01.100
- Onyilagha, J.C., Gruber, M.Y., Hallett, R.H., Holowachuk, J., Buckner, A., et Soroka, J.J. (2012). « Constitutive flavonoids deter flea beetle insect feeding in Camelina sativa L. », Biochemical Systematics and Ecology, 42, p. 128-133. doi : 10.1016/j.bse.2011.12.021
- Salah, I.B., Jelali, N., Slatni, T., Gruber, M.Y., Albacete, A., Andújar, C.M., Martinez, V., Perez-Alfocea, F., et Abdelly, C. (2012). « Involvement of source-sink relationship and hormonal control in the response of Medicago ciliaris - Sinorhizobium medicae symbiosis to salt stress. », Acta Biologica Hungarica, 63(1), p. 97-112. doi : 10.1556/ABiol.63.2012.1.8
- Steppuhn, H., Acharya, S.N., Iwaasa, A.D., Gruber, M.Y., et Miller, D.R. (2012). « Réaction inhérente de neuf populations de luzerne à la salinité du sol dans la zone racinaire. », Canadian Journal of Plant Science, 92(2), p. 235-248. doi : 10.4141/CJPS2011-174
- Taheri, A., Robinson, S.J., Parkin, I.A.P., et Gruber, M.Y. (2012). « Revised Selection Criteria for Candidate Restriction Enzymes in Genome Walking. », PLoS ONE, 7(4, Article No. e35117). doi : 10.1371/journal.pone.0035117
- Wei, S., Gruber, M.Y., Yu, B., Gao, M.-J., Khachatourians, G.G., Hegedus, D.D., Parkin, I.A.P., et Hannoufa, A. (2012). « Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network. », BMC Plant Biology, 12:169. doi : 10.1186/1471-2229-12-169
- Yu, B., Gruber, M.Y., Khachatourians, G.G., Zhou, R., Epp, D.J., Hegedus, D.D., Parkin, I.A.P., Welsch, R., et Hannoufa, A. (2012). « Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus. », Journal of Experimental Botany, 63(14), p. 5189-5202. doi : 10.1093/jxb/ers179
2011 :
- Ben Salah, I., Mahmoudi, H., Gruber, M.Y., Slatni, T., Boulaaba, M., Gandour, M., Messedi, D., Hamed, K.B., Ksouri, R., Hannoufa, A., et Abdelly, C. (2011). « Phenolic content and antioxidant activity in two contrastin Medicago ciliaris lines cultivated under salt stress. », Biologia, 66(5), p. 813-820. doi : 10.2478/s11756-011-0102-6
- Ben Salah, I., Slatni, T., Gruber, M.Y., Mahmoudi, H., Zribi, K., et Abdelly, C. (2011). « Variability in the response of six genotypes of N2-fixing Medicago ciliaris to NaCl. », Symbiosis, 53(3), p. 139-147. doi : 10.1007/s13199-011-0118-2
- Ben Salah, I., Slatni, T., Gruber, M.Y., Messedi, D., Gandour, M., Benzarti, M., Haouala, R., Zribi, K., Hamed, K.B., Perez-Alfocea, F., et Abdelly, C. (2011). « Relationship between symbiotic nitrogen fixation, sucrose synthesis and anti-oxidant activities in source leaves of two Medicago ciliaris lines cultivated under salt stress. », Environmental and Experimental Botany, 70(2-3), p. 166-173. doi : 10.1016/j.envexpbot.2010.08.014
- Hossain, Z., McGarvey, B.D., Macfie, S. M., Amyot, L., Gruber, M.Y., Jung, J., Hannoufa, A. (2011). « DIMINUTO 1 affects the lignin profile and secondary cell wall formation in Arabidopsis. », Planta, 235(3), p. 485-498. doi : 10.1007/s00425-011-1519-4
- Jonker, A., Gruber, M.Y., Wang, Y., Coulman, B.E., Azarfar, A., McKinnon, J.J., Christensen, D.A., et Yu, P. (2011). « Modeling degradation ratios and nutrient availability of anthocyanidin-accumulating Lc-alfalfa populations in dairy cows. », Journal of Dairy Science (JDS), 94(3), p. 1430-1444. doi : 10.3168/jds.2010-3604
- Kaddour, R., Mahmoudi, H., Baâtour, Olfa, Tarchoune, I., Nasri, N., Ben Salah, I., Berthomieu, P., Gruber, M.Y., et Mokhtar, L. (2011). « Physiological and molecular responses of two Arabidopsis accessions to calcium amendment and salt constraint. », Acta Physiologiae Plantarum, 34(2), p. 439-450. doi : 10.1007/s11738-011-0840-7
- Li, X.L., Gao, P., Cui, D., Wu, L., Parkin, I.A.P., Saberianfar, R., Menassa, R., Pan, H.-Y., Westcott, N.D., et Gruber, M.Y. (2011). « The Arabidopsis tt19-4 mutant differentially accumulates proanthocyanidin and anthocyanin through a 3′ amino acid substitution in glutathione S-transferase. », Plant, Cell & Environment, 34(3), p. 374-388. doi : 10.1111/j.1365-3040.2010.02249.x
- Li, X.L., Gruber, M.Y., Hegedus, D.D., Lydiate, D.J., et Gao, M.-J. (2011). « Effects of a Coumarin Derivative, 4-Methylumbelliferone, on Seed Germination and Seedling Establishment in Arabidopsis. », Journal of Chemical Ecology, 37(8), p. 880-890. doi : 10.1007/s10886-011-9987-3
- Li, X.L., Qin, J.-C., Wang, Q.-Y., Wu, X., Lang, C.-Y., Pan, H.-Y., Gruber, M.Y., et Gao, M.-J. (2011). « Metabolic engineering of isoflavone genistein in Brassica napus with soybean isoflavone synthase. », Plant Cell Reports, 30(8), p. 1435-1442. doi : 10.1007/s00299-011-1052-8
- Soroka, J.J., Holowachuk, J., Gruber, M.Y., et Grenkow, L.F. (2011). « Feeding by Flea Beetles (Coleoptera: Chrysomelidae; Phyllotreta spp.) Is Decreased on Canola (Brassica napus) Seedlings with Increased Trichome Density. », Journal of Economic Entomology, 104(1), p. 125-136. doi : 10.1603/EC10151
- Wei, S., Hannoufa, A., Soroka, J.J., Xu, N., Li, X.L., Gruber, M.Y., et Zebarjadi, A. (2011). « Enhanced ß-ionone Emission in Arabidopsis Over-Expressing AtCCD1 Reduces Feeding Damage in vivo by the Crucifer Flea Beetle. », Environmental Entomology, 40(6), p. 1622-1630. doi : 10.1603/EN11088
- Xiong, J., Yang, Q., Kang, J., Sun, Y., Zhang, T., Gruber, M.Y., et Ding, W. (2011). « Simultaneous isolation of DNA, RNA, and protein from Medicago truncatula L. », Electrophoresis, 32(2), p. 321-330. doi : 10.1002/elps.201000425
2010 :
- Gao, P., Li, X.L., Cui, D., Wu, L., Parkin, I.A.P., et Gruber, M.Y. (2010). « A new dominant Arabidopsis transparent testa mutant, sk21-D, and modulation of seed flavonoid biosynthesis by KAN4. », Plant Biotechnology Journal, 8(9), p. 979-993. doi : 10.1111/j.1467-7652.2010.00525.x
- Jonker, A., Gruber, M.Y., McCaslin, M., Wang, Y., Coulman, B.E., McKinnon, J.J., Christensen, D.A., et Yu, P. (2010). « Composition nutritive et profil de dégradation de la luzerne-Lc accumulant l’anthocyanidine. », Canadian Journal of Animal Science, 90(3), p. 401-412. doi : 10.4141/CJAS09110
- Li, X.L., Gao, M.-J., Pan, H.-Y., Cui, D., et Gruber, M.Y. (2010). « Purple canola: Arabidopsis PAP1 increases antioxidants and phenolics in brassica napus leaves. », Journal of Agricultural and Food Chemistry, 58(3), p. 1639-1645. doi : 10.1021/jf903527y
- Li, X.L., Westcott, N.D., Links, M.G., et Gruber, M.Y. (2010). « Seed Coat Phenolics and the Developing Silique Transcriptome of Brassica carinata. », Journal of Agricultural and Food Chemistry, 58(20), p. 10918-10928. doi : 10.1021/jf102208a
- Mahmoudi, H., Huang, A.J., Gruber, M.Y., Kaddour, R., Mokhtar, L., Zeineb, O., et Hannoufa, A. (2010). « The Impact of Genotype and Salinity on Physiological Function, Secondary Metabolite Accumulation, and Antioxidative Responses in Lettuce. », Journal of Agricultural and Food Chemistry, 58(8), p. 5122-5130. doi : 10.1021/jf904274v
- Wei, S., Yu, B., Gruber, M.Y., Khachatourians, G.G., Hegedus, D.D., et Hannoufa, A. (2010). « Enhanced seed carotenoid levels and branching in transgenic Brassica napus expressing the Arabidopsis miR156b gene. », Journal of Agricultural and Food Chemistry, 58(17), p. 9572-9578. doi : 10.1021/jf102635f
- Yu, B., Gruber, M.Y., Khachatourians, G.G., Hegedus, D.D., et Hannoufa, A. (2010). « Gene expression profiling of developing Brassica napus seed in relation to changes in major storage compounds. », Plant Science, 178(4), p. 381-389. doi : 10.1016/j.plantsci.2010.02.007
2009 :
- Gruber, M.Y., Xu, N., Grenkow, L.F., Onyilagha, J.C., Soroka, J.J., Westcott, N.D., et Hegedus, D.D. (2009). « Responses of the crucifer flea beetle to brassica volatiles in an olfactometer. », Environmental Entomology, 38(5), p. 1467-1479. doi : 10.1603/022.038.0515
- Li, X.L., Gao, P., Gjetvaj, B., Westcott, N.D., et Gruber, M.Y. (2009). « Analysis of the metabolome and transcriptome of Brassica carinata seedlings after lithium chloride exposure. », Plant Science, 177(1), p. 68-80. doi : 10.1016/j.plantsci.2009.03.013
- Robinson, S.J., Tang, L.H., Mooney, B.G., McKay, S.J., Clarke, W.E., Links, M.G., Karcz, S.R., Regan, S., Wu, Y.-Y., Gruber, M.Y., Cui, D., Yu, M., et Parkin, I.A.P. (2009). « An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches. », BMC Plant Biology, 9:101. doi : 10.1186/1471-2229-9-101
- Wei, S., Li, X.L., Gruber, M.Y., Zhou, R., Zebarjadi, A., et Hannoufa, A. (2009). « RNAi-Mediated Suppression of DET1 Alters the Levels of Carotenoids and Sinapate Esters in Seeds of Brassica napus. », Journal of Agricultural and Food Chemistry, 57(12), p. 5326-5333. doi : 10.1021/jf803983w
- Yu, P., Jonker, A., et Gruber, M.Y. (2009). « Molecular basis of protein structure in proanthocyanidin and anthocyanin-enhanced Lc-transgenic alfalfa in relation to nutritive value using synchrotron-radiation FTIR microspectroscopy: A novel approach. », Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 73(5), p. 846-853. doi : 10.1016/j.saa.2009.04.006
Autres publications relatives à la science :
2012 :
- Li, X., Gruber, M.Y., Falk, K.C., et Westcott, N.D. (2012). « Phytoremediation capacity of brown-seeded and yellow-seeded Brassica carinata. », dans Anjum, N.A., Pereira, M.E., Ahmad, I., Duarte, A.C., Umar S., and Khan, N.A. (dir.) - Phytotechnologies: Remediation of Environmental Contaminants, CRC Press Taylor & Francis.
2011 :
- Alahakoon, U.I., Bonham-Smith, P.C., et Gruber, M.Y. (2011). « New Hairy Canola line and its potential for resistance to Phyllotreta flea beetles. », Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011, CSA-S16, p. 200.
- Alahakoon, U.I., Gruber, M.Y., et Bonham-Smith, P.C. (2011). « A new hairy canola line and its potential for resistance to crucifer flea beetles. », University of Saskatchewan, Saskatchewan, SK, Canada, October 21, 2011. (Présentation)
- Gruber, M.Y. (2011). « Hairy Canola - Flea Beetle’s Downfall. », Gardenscape, Entomological Society of Saskatchewan booth, Saskatoon, SK, Canada, March 25, 2011. (Présentation)
- Gruber, M.Y. (2011). « Hairy Canola - Flea Beetle’s Downfall. », Western Canadian Crop Production Show, Exhibition Centre, Saskatoon, SK, Canada, January 11, 2011. (Présentation)
- Gruber, M.Y. (2011). « Trichome modulation leads to flea beetle resistance in Brassica napus. », Canola Council of Canada, January 19, 2011. (Présentation)
- Gruber, M.Y. et Soroka, J.J. (2011). « Quand les altises des crucifères rencontrent le colza velu. », publié par Agriculture and Agri-Food Canada (AAFC)/Agriculture et Agroalimentaire Canada (AAC).
- Gruber, M.Y., Li, X., Gao, P., Cui, D., Yu, M., Nayidu, N., Alahakoon, U.I., Wei, S., Yu, B., Holowachuk, J., Bonham-Smith, P.C., Regan, S., Hannoufa, A., Hossein, Z., et Parkin, I.A.P. (2011). « Unravelling Mysteries in the Regulation of Plant Secondary Metabolites and Non-glandular Trichomes: New Mechanisms and Crop Applications. », 50th Anniversary Meeting of the Phytochemical Society of North America, Fairmont Orchid Hotel and HILO Conference Centre, Kohala Coast, Big Island, HI, USA, December 10-15, 2011. (Présentation)
- Hannoufa, A., Wei, S., Gruber, M.Y., Khachatourians, G.G., Wang, Y., Hegedus, D.D., et Parkin, I.A.P. (2011). « MicroRNA156: A new player in regulating carotenoid accumulation in plants. », Canadian Society of Plant Physiologists Eastern Regional Meeting & Plant Development Workshop / Congrès de la Société Canadienne de Physiologie Végétale & Congrès de Développement Végétale (Congrès Régional de l’Est), Carleton University, Ottawa, ON, Canada, December 2-3, 2011, SB-1, p. 17.
- Nayidu, N., Li, X., et Gruber, M.Y. (2011). « Study of Brassica villosa trichomes and trichome-related genes. », St. Mary’s University, Halifax, NS, Canada, July 8-11, 2011, CSPP-20, p. 301. (Affiche)
2010 :
- Bittman, S. et Gruber, M.Y. (2010). « Cellulosic Biofuel Crop production and nutrient cycling: Activities and some preliminary results. », Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #28, p. 31. (Affiche)
- Gruber, M.Y. (2010). « Biomass Enhancement: Research in the Canadian Cellulosic Biofuel Network. », Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #8, p. 21. (Affiche)
- Gruber, M.Y. (2010). « Biomass Production: Research in the Canadian Cellulosic Biofuel Network. », Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #9, p. 21. (Affiche)
- Gruber, M.Y. (2010). « Physico-chemical pre-treatment and composition of Biomass in the Canadian Cellulosic Biofuel Network. », Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #10, p. 22. (Affiche)
- Li, X.L., Holowachuk, J., Murthy, R.K., Bouatra, S., Sinelnikov, I., Mandal, R., Wishart, D.S., Westcott, N.D., et Gruber, M.Y. (2010). « Tolerance of ethiopian mustard to sodium sulphate salinity. », Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #29, p. 31. (Affiche)
- Papadopoulos, Y.A., Glover, K., Gruber, M.Y., Fillmore, S.A.E., et Thomas, B. (2010). « The evaluation of newly developed Alfalfa varieties for adaptability under fall and spring waterlogging. », Cellulosic Biofuel Network (CBN) 2nd Annual General Meeting, Ottawa Marriott Hotel, Ottawa, ON, Canada, March 11-12, 2010, Poster #27, p. 30. (Affiche)
2009 :
- Gruber, M.Y. et Ray, H. (2009). « Novel regulatory genes involved in condensed tannin synthesis in plants. », USA Patent 7531717, May 12, 2009. (Brevet)
- Gruber, M.Y., Ray, H., et Westcott, N.D. (2009). « Regulation of flavonoid expression in alfalfa using maize regulatory genes. », USA Patent 7521600, April 21, 2009. (Brevet)
- Zhou, R., Tsang, E., Fu, Y.B., et Gruber, M.Y. (2009). « Rapid determination of S-Adenosylmethionine and S-Adenosylhomocysteine in plant tissues by UPLC-Tandem Mass Spectrometry. », 5th International Conference of the Metabolomics Society, Crowne Plaza Chateau Lacombe, Edmonton, AB, Canada, August 30-September 2, 2009. (Affiche)