Marsolais, Frédéric, Ph.D.
Research Scientist, Protein ChemistryContact Information:
1391 Sandford St
London, Ontario N5V 4T3
Telephone: 519-457-1470 (311)
Fax: 519-457-3997
Email: frederic.marsolais@agr.gc.ca
Areas of Expertise:
- protein chemistry of pulses
- plant nitrogen metabolism
- amino acid metabolism
- protein biochemistry
- molecular genetics
- enzymology
- proteolytic processing
Current Projects:
- Molecular characterization of two cytokinin-inducible cytochrome P450s from Arabidopsis.
- Regulation of N supply and assimilation in developing seeds of common bean (Phaseolus vulgaris).
- Proteomic analysis of seed protein composition in common bean (Phaseolus vulgaris)
Affiliations:
- Adjunct Professor, University of Western Ontario, Department of Biology
Research Publications:
The links below lead to abstracts with access to full text.2013:
- Liao, D., Cram, D., Sharpe, A.G., and Marsolais, F. (2013). "Transcriptome profiling identifies candidate genes associated with the accumulation of distinct sulfur γ-glutamyl dipeptides in Phaseolus vulgaris and Vigna mungo seeds.", Frontiers in Plant Science, 4:60. doi : 10.3389/fpls.2013.00060
- Lin, Y., Pajak, A., Marsolais, F., McCourt, P., and Riggs, C.D. (2013). "Characterization of a cruciferin deficient mutant of Arabidopsis and its utility for overexpression of foreign proteins in plants.", PLoS ONE, 8(5), pp. e64980. doi : 10.1371/journal.pone.0064980
- Zhang, Q., Lee, J., Pandurangan, S., Clarke, M., Pajak, A., and Marsolais, F. (2013). "Characterization of Arabidopsis serine: glyoxylate aminotransferase, AGT1, as an asparagine aminotransferase.", Phytochemistry, 85, pp. 30-35. doi : 10.1016/j.phytochem.2012.09.017
2012:
- Hagel, J.M., Krizevski, R., Marsolais, F., Lewinsohn, E., and Facchini, P.J. (2012). "Biosynthesis of amphetamine analogues in plants.", Trends in Plant Science, 17(7), pp. 404-412. doi : 10.1016/j.tplants.2012.03.004
- Ivanov, A., Kameka, A., Pajak, A., Bruneau, L., Beyaert, R.P., Hernández-Sebastià, C., and Marsolais, F. (2012). "Arabidopsis mutants lacking asparaginases develop normally but exhibit enhanced root inhibition by exogenous asparagine.", Amino Acids, 42(6), pp. 2307-2318. doi : 10.1007/s00726-011-0973-4
- Krizevski, R., Bar, E., Shalit, O., Levy, A., Hagel, J.M., Kilpatrick, K., Marsolais, F., Facchini, P.J., Ben-Shabat, S., Sitrit, Y., and Lewinsohn, E. (2012). "Benzaldehyde is a precursor of phenylpropylamino alkaloids as revealed by targeted metabolic profiling and comparative biochemical analyses in Ephedra spp.", Phytochemistry, 81, pp. 71-79. doi : 10.1016/j.phytochem.2012.05.018
- Liao, D., Pajak, A., Karcz, S.R., Chapman, B. P., Austin, R.S., Sharpe, A.G., Datla, R.S.S., Dhaubhadel, S., and Marsolais, F. (2012). "Transcripts of sulfur metabolic genes are coordinately regulated in developing seeds of common bean lacking phaseolin and major lectins.", Journal of Experimental Botany, 63(17), pp. 6283-6295. doi : 10.1093/jxb/ers280
- Liu, W., Liang, Z., Shan, C.-J., Marsolais, F., and Tian, L.-N. (2012). "Genetic transformation and full recovery of alfalfa plants via secondary somatic embryogenesis.", In Vitro Cellular & Developmental Biology - Plant, 49(1), pp. 17-23. doi : 10.1007/s11627-012-9463-y
- Pandurangan, S., Pajak, A., Molnar, S.J., Cober, E.R., Dhaubhadel, S., Hernández-Sebastià, C., Kaiser, W.M., Nelson, R.L., Huber, S.C., and Marsolais, F. (2012). "Relationship between asparagine metabolism and protein concentration in soybean seed.", Journal of Experimental Botany, 63(8), pp. 3173-3184. doi : 10.1093/jxb/ers039
2011:
- Gabriel, M., Telmer, P.G., and Marsolais, F. (2011). "Role of asparaginase variable loop at the carboxyl terminal of the alpha subunit in the determination of substrate preference in plants.", Planta, 235(5), pp. 1013-1022. doi : 10.1007/s00425-011-1557-y
- Hagel, J.M., Krizevski, R., Kilpatrick, K., Sitrit, Y., Marsolais, F., Lewinsohn, E., and Facchini, P.J. (2011). "Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids.", Genetics and Molecular Biology, 34, pp. 640-646.
- Yin, F., Pajak, A., Chapman, R.A., Sharpe, A.G., Huang, S.Z., and Marsolais, F. (2011). "Analysis of common bean expressed sequence tags identifies sulfur metabolic pathways active in seed and sulfur-rich proteins highly expressed in the absence of phaseolin and major lectins.", BMC Genomics, 12:268. doi : 10.1186/1471-2164-12-268
2010:
- Dhaubhadel, S., Marsolais, F., Tedman-Jones, J., and Gijzen, M. (2010). "Functional Genomics - Transcriptomics in Soybean.", in Bilyeu, K., Ratnaparkhe, M., and Kole, C. (eds.) - Genetics, Genomics and Breeding of Soybean. Series: Genetics, Genomics and Breeding of Crop Plants, Science Publishers and CRC Press, Chapter 9, pp. 199-222.
- Marsolais, F., Pajak, A., Yin, F., Taylor, M., Gabriel, M., Merino, D.M., Ma, V., Kameka, A., Vijayan, P., Pham, H., Huang, S.Z., Rivoal, J., Bett, K.E., Hernández-Sebastià, C., Liu, Q., Bertrand, A., and Chapman, R.A. (2010). "Proteomic analysis of common bean seed with storage protein deficiency reveals up-regulation of sulfur-rich proteins and starch and raffinose metabolic enzymes, and down-regulation of the secretory pathway.", Journal of Proteomics, 73(8), pp. 1587-1600. doi : 10.1016/j.jprot.2010.03.013
- Yi, J., Derynck, M.R., Li, X., Telmer, P.G., Marsolais, F., and Dhaubhadel, S. (2010). "A single repeat MYB transcription factor, GmMYB176, regulates CHS8 gene expression and affects isoflavonoid biosynthesis in soybean.", Plant Journal, 62(6), pp. 1019-1034. doi : 10.1111/j.1365-313X.2010.04214.x
Other Science Related Publications:
2012:
- Bejger, M., Imiolczyk, B., and Marsolais, F. (2012). "The first crystal structure of potassium-dependent Ntn-hydrolase. Oral presentation.", 15th Heart of Europe Biocrystallography Meeting (HEC-15), Beilngries, Germany, September 28-30, 2012.
- Dhaubhadel, S. and Marsolais, F. (2012). "Transcriptomics of Legume Seed: Soybean a Model Grain Legume.", in Agrawal, G.K. and Rakwal, R. (eds.) - Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. OMICS in Seed Biology, Springer, Chapter 8, pp. 129-142.
- Liao, D., Pajak, A., and Marsolais, F. (2012). "Transcripts of sulfur metabolic genes are coordinately regulated by protein demand in seed lacking phaseolin and major lectins.", Phaseomics – The Genome Meeting, Guanajuato, México, June 21-23, 2012. (Presentation)
- Pandurangan, S. and Marsolais, F. (2012). "Genomic analysis of storage protein deficient lines of common bean.", 9th Canadian Pulse Research Workshop, Niagara Falls Marriott Gateway, Niagara Falls, ON, Canada, November 6-12, 2012. (Poster)
- Pandurangan, S., Pajak, A., Austin, R.S., Gilski, M., Marsolais, F., and Jaskolski, M. (2012). "Transcriptional responses to exogenous asparagine in Arabidopsis roots.", 51st Phytochemical Society of North America (PSNA) Annual Meeting, University of Western Ontario, London, ON, Canada, August 11-15, 2012, Published in: Pharm. Biol., (50), pp. 1341.
- Perry, G.E., Crosby, W.L., Yu, K., Navabi, A., Zou, X., Power, K.A., Marsolais, F., Xie, W., Weersink, A., Tsao, R., Cooper, D., and Pauls, K.P. (2012). "Sequencing the bean genome: Using comparative genomics to examine interspecific introgressions.", Canadian Society of Plant Biologists Meeting (CSPB 2012), University of Alberta Campus, Edmonton, AB, Canada, June 25-27, 2012. (Presentation)
- Schernthaner, J.P., Singh, J., Labbé, N.M., Liao, D., and Marsolais, F. (2012). "The unfolded protein response in plant seeds.", in Hendriks, B.P. (ed.) - Agricultural Research Updates. Vol. 2, Nova Science Publishers Inc, pp. 429-454.
- Zhang, Q., Pajak, A., and Marsolais, F. (2012). "Identification and characterization of AGT1 as an asparagine aminotransferase in Arabidopsis thaliana. Poster presentation.", Canadian Society of Plant Biologists Meeting (CSPB 2012), University of Alberta Campus, Edmonton, AB, Canada, June 25-27, 2012.
2011:
- Kilpatrick, K., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2011). "Functional genomics investigation of aminotransferases involved in ephedrine alkaloid biosynthesis in Ephedra sinica Stapf.", 8th Canadian Plant Genomics Workshop, Niagara Falls, ON, Canada, August 22-25, 2011. (Presentation)
- Kilpatrick, K., Krizevski, R., Hagel, J.M., Lewinsohn, E., Facchini, P.J., and Marsolais, F. (2011). "Functional genomics investigation of aromatic aminotransferase enzymes involved in ephedrine alkaloid biosynthesis.", 2nd Annual Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 22, 2011. (Poster)
- Lee, J. and Marsolais, F. (2011). "Genetic characterization of the response to exogenous asparagine in asparaginase double knock-out mutants of Arabidopsis thaliana seedlings.", Ontario Biology Day, Wilfrid Laurier University, Waterloo, ON, Canada, March 12-13, 2011.
- Perry, G.E., Crosby, W., Shi, C., Power, K.A., Marsolais, F., Xie, W., Weersink, A., Tsao, R., Cooper, D.M., and Pauls, K.P. (2011). "Sequencing the bean genome: the applied bean genomics and bioproducts project.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011. (Poster)
- Perry, G.E., Crosby, W., Yu, K., Shi, C., Powers, K., Marsolais, F., Xie, W., Weersink, A., Tsao, R., Siddiqua, M., Cooper, D.M., and Pauls, K.P. (2011). "The applied bean genomics and bioproducts project: sequencing the bean genome.", Biennial Meeting of the Bean Improvement Cooperative and North American Pulse Improvement Association (BIC/NAPIA 2011), Verdanza Hotel, San Juan, Puerto Rico, October 30-November 2, 2011.
- Zhang, Q. and Marsolais, F. (2011). "Metabolism and transcriptional responses to asparagine in Arabidopsis thaliana.", 2nd Annual Biology Graduate Research Forum, University of Western Ontario, London, ON, Canada, October 22, 2011. (Poster)
2009:
- Ivanov, A.G. and Marsolais, F. (2009). "Functional characterization and regulation of asparaginases in Arabidopsis. Poster presentation.", 2009 Annual Meeting of the Canadian Society of Plant Physiologists, Simon Fraser University, Burnaby, BC, Canada, June 12-15, 2009.
- Marsolais, F., Pajak, A., Yin, F., Huang, S.Z., and Chapman, R.A. (2009). "Proteomic Analysis of Seed Storage Protein Deficiency in Common Bean.", Phaseomics VI Meeting, Palace of Salcedo, Pontevedra, Spain, May 21-23, 2009, Actas de la Asociación Española de Leguminosas 4, pp. 53.