Lévesque, André, Ph.D.
Research Scientist, mycologyContact Information:
960 Carling Ave
Ottawa, Ontario K1A 0C6
Telephone: 613-759-1579
Fax: 613-759-1701
Email: andre.levesque@agr.gc.ca
Areas of Expertise:
- mycology
- molecular systematics of zoosporic fungi, with an emphasis on Oomycetes and Pythium
- comparative genomics
- plant pathology
- detection of fungi and disease diagnostics
- herbicide-fungi interactions
Current Projects:
- Build and maintain reference collections and databases of fungi [AAFC National Mycological Herbarium (DAOM) and Canadian Collection of Fungal Cultures (CCFC)]
- Development of phylogenomics capability, rapid detection and identification tools
- Develop new information on taxonomy, phylogeny, distribution and biology of fungi, including systematic research related to biosecurity/alien invasive species as well as species involved in the development of bioproducts
Research Publications:
The links below lead to abstracts with access to full text.2013:
- Schroeder, K.L>, Martin, F.N., de Cock, A.W.A.M., Lévesque, C.A., Spies, C.F.J., Okubara, P.A., and Paulitz, T.C. (2013). "Molecular detection and quantification of Pythium species: Evolving taxonomy, new tools, and challenges.", Plant Disease, 97(1), pp. 4-20. doi : 10.1094/pdis-03-12-0243-fe
2012:
- Chen, W., Robleh Djama, Z., Coffey, M.D., Martin, F.N., Bilodeau, G.J., Radmer, L., Denton, G., and Lévesque, C.A. (2012). "Membrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species.", Phytopathology, 103(1), pp. 43-54. doi : 10.1094/PHYTO-04-12-0092-R
- Rintoul, T.L., Eggertson, Q.A., and Lévesque, C.A. (2012). "Multigene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens.", Methods in Molecular Biology (Clifton, N.J.), 835, pp. 549-569. doi : 10.1007/978-1-61779-501-5_34
- Schoch, C.L., Seifert, K.A., Huhndorf, S.M., Robert, V.A., Spouge, J.L., Lévesque, C.A., Chen, W., and Fungal Barcoding Consortium, (2012). "Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi.", Proceedings of the National Academy of Sciences of the USA (PNAS), 109(16), pp. 6241-6246. doi : 10.1073/pnas.1117018109
2011:
- Hamilton, J.P., Neeno-Eckwall, E.C., Adhikari, B.N., Perna, N.T., Tisserat, N., Leach, J.E., Lévesque, C.A., and Buell, C.R. (2011). "The Comprehensive Phytopathogen Genomics Resource: a web-based resource for data-mining plant pathogen genomes.", Database: The Journal of Biological Databases and Curation, 2011(Article ID bar053). doi : 10.1093/database/bar053
- Hawksworth, D.L., Crous, P.W., Redhead, S.A., Reynolds, D.R., Samson, R.A., Seifert, K.A., Taylor, J.W., Wingfield, M.J., Abaci, Ö., Aime, C., Asan, A., Bai, F.-Y., de Beer, Z.W., Begerow, D., Berikten, D., Boekhout, T., Buchanan, P.K., Burgess, T.I., Buzina, W., Cai, L., Cannon, P.F., Crane, J.L., Damm, U., Daniel, H.-M., van Diepeningen, A.D., Druzhinina, I.S., Dyer, P.S., Eberhardt, U., Fell, J.W., Frisvad, J.C., Geiser, D.M., Geml, J., Glienke, C., Gräfenhan, T., Groenewald, J.Z., Groenewald, M., de Gruyter, J.Z., Guého-Kellermann, E., Hibbett, D.S., Hong, S.-B., de Hoog, G.S., Houbraken, J.A.M.P., Huhndorf, S.M., Hyde, K.D., Ismail, A., Johnston, P.R., Kadaifciler, D.G., Kirk, P.M., Kõljalg, U., Kurtzman, C.P., Lagneau, P.-E., Lévesque, C.A., and others (2011). "The Amsterdam Declaration on Fungal Nomenclature.", IMA Fungus, 2(1), pp. 105-112. doi : 10.5598/imafungus.2011.02.01.14
- Lévesque, C.A. (2011). "Fifty years of oomycetes - from consolidation to evolutionary and genomic exploration.", Fungal Diversity, 50(1), pp. 35-46. doi : 10.1007/s13225-011-0128-7
- Lewis, C.T., Bilkhu, S., Robert, V.A., Eberhardt, U., Szöke, S., Seifert, K.A., and Lévesque, C.A. (2011). "Identification of Fungal DNA Barcode Targets and PCR Primers Based on Pfam Protein Families and Taxonomic Hierarchy.", Open Applied Informatics Journal, 5(Suppl. 1-M5), pp. 30-44. doi : 10.2174/1874136301105010030
- Robert, V.A., Szöke, S., Eberhardt, U., Cardinali, G., Meyer, W., Seifert, K.A., Lévesque, C.A., and Lewis, C.T. (2011). "The Quest for a General and Reliable Fungal DNA Barcode.", Open Applied Informatics Journal, 5(Suppl. 1-M6), pp. 45-61.
- Robideau, G.P., de Cock, A.W.A.M., Coffey, M.D., Voglmayr, H., Brouwer, H., Bala, K., Chitty, D.W., Désaulniers, N.L., Eggertson, Q.A., Gachon, C.M.M., Hu, C.-H., Küpper, F.C., Rintoul, T.L., Sarhan, E., Verstappen, E.C.P., Zhang, Y., Bonants, P.J.M., Ristaino, J.B., and Lévesque, C.A. (2011). "DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer.", Molecular Ecology Resources, 11(6), pp. 1002-1011. doi : 10.1111/j.1755-0998.2011.03041.x
- Tsui, C.K.M., Woodhall, J., Chen, W., Lévesque, C.A., Lau, A., Schoen, C.D., Baschien, C., Najafzadeh, M.J., and de Hoog, G.S. (2011). "Molecular techniques for pathogen identification and fungus detection in the environment.", IMA Fungus, 2(2), pp. 177-189. doi : 10.5598/imafungus.2011.02.02.09
2010:
- Abbott, C.L., Gilmore, S.R., Lewis, C.T., Chapados, J.T., Peters, R.D., Platt, H.W., Coffey, M.D., and Lévesque, C.A. (2010). "Development of a SNP genetic marker system based on variation in microsatellite flanking regions of Phytophthora infestans.", Canadian Journal of Plant Pathology / Revue canadienne de phytopathologie, 32(4), pp. 440-457. doi : 10.1080/07060661.2010.510648
- Bala, K., Robideau, G.P., Désaulniers, N.L., de Cock, A.W.A.M., and Lévesque, C.A. (2010). "Taxonomy, DNA barcoding and phylogeny of three new species of Pythium from Canada.", Persoonia, 25, pp. 22-31. doi : 10.3767/003158510X524754
- Bala, K., Robideau, G.P., Lévesque, C.A., de Cock, A.W.A.M., Abad, G., Lodhi, M.A., Shahzad, S., Ghaffar, A., and Coffey, M.D. (2010). "Phytopythium Abad, de Cock, Bala, Robideau, Lodhi & Lévesque, gen. nov. and Phytopythium sindhum Lodhi, Shahzad & Lévesque, sp. nov. Fungal Planet 49.", Persoonia, 24, pp. 136-137.
- Demeke, T., Gräfenhan, T., Clear, R.M., Phan, A., Ratnayaka, I., Chapados, J.T., Patrick, S.K., Gaba, D., and Seifert, K.A., and Lévesque, C.A. (2010). "Development of a specific TaqMan® real-time PCR assay for quantification of Fusarium graminearum clade 7 and comparison of fungal biomass determined by PCR with deoxynivalenol content in wheat and barley.", International Journal of Food Microbiology, 141(1-2), pp. 45-50. doi : 10.1016/j.ijfoodmicro.2010.04.020
- Lévesque, C.A., Brouwer, H., Cano, L., Hamilton, J.P., Holt, C., Huitema, E., Raffaele, S., Robideau, G.P., Thines, M., Win, J., Zerillo, M.Z., Beakes, G.W., Boore, J.L., Busam, D., Dumas, B., Ferriera, S., Fuerstenberg, S.I., Gachon, C.M.M., Gaulin, E., Govers, F., Grenville-Briggs, L., Horner, N., Hostetler, J., Jiang, R.H.Y., Johnson, J., Krajaejun, T., Lin, H., Meijer, H.J.G., Moore, B., Morris, P.F., Phuntumart, V., Puiu, D., Shetty, J., Stajich, J.E., Tripathy, S., Wawra, S., van West, P., Whitty, B.R., Coutinho, P.M., Henrissat, B., Martin, F.N., Thomas, P.D., Tyler, B.M., De Vries, R.P., Kamoun, S., Yandell, M., Tisserat, N., and Buell, C.R. (2010). "Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire.", Genome Biology, 11(R73). doi : 10.1186/gb-2010-11-7-r73
- Pfrender, M.E., Hawkins, C.P., Bagley, M., Courtney, G.W., Creutzburg, B.R., Epler, J.H., Fend, S., Ferrington Jr., L.C., Hartzell, P.L., Jackson, S., Larsen, D.P., Lévesque, C.A., Morse, J.C., Petersen, M.J., Ruiter, D., Schindel, D., and Whiting, M. (2010). "Assessing macroinvertebrate biodiversity in freshwater ecosystems: advances and challenges in DNA-based approaches.", Quarterly Review of Biology, 85(3), pp. 319-340. doi : 10.1086/655118
- Sokolski, S., Dalpé, Y., Séguin, S.M., Khasa, D.P., Lévesque, C.A., and Piché, Y. (2010). "Conspecificity of DAOM 197198, the model arbuscular mycorrhizal fungus, with Glomus irregulare: molecular evidence with three protein-encoding genes.", Botany / Botanique, 88(9), pp. 829-838. doi : 10.1139/B10-050
2009:
- Bilodeau, G.J., Pelletier, G., Pelletier, F., Lévesque, C.A., and Hamelin, R.C. (2009). "Multiplex real-time polymerase chain reaction (PCR) for detection of Phytophthora ramorum, the causal agent of sudden oak death.", Canadian Journal of Plant Pathology / Revue canadienne de phytopathologie, 31(2), pp. 195-210. doi : 10.1080/07060660909507593
- Carisse, O., Tremblay, D.M., Lévesque, C.A., Gindro, K., Ward, P., and Houde, A. (2009). "Development of a TaqMan real-time PCR assay for quantification of airborne conidia of Botrytis squamosa and management of Botrytis leaf blight of onion.", Phytopathology, 99(11), pp. 1273-1280. doi : 10.1094/PHYTO-99-11-1273
- Chen, W., Seifert, K.A., and Lévesque, C.A. (2009). "A high density COX1 barcode oligonucleotide array for identification and detection of species of Penicillium subgenus Penicillium.", Molecular Ecology Resources, 9(Suppl. 1), pp. 114-129. doi : 10.1111/j.1755-0998.2009.02638.x
- Senda, M., Kageyama, K., Suga, H., and Lévesque, C.A. (2009). "Two new species of Pythium, P. senticosum and P. takayamanum, isolated from cool-temperate forest soil in Japan.", Mycologia, 101(4), pp. 439-448. doi : 10.3852/08-104
- Vallance, J., Le Floch, G., Déniel, F., Barbier, G., Lévesque, C.A., and Rey, P. (2009). "Influence of Pythium oligandrum Biocontrol on Fungal and Oomycete Population Dynamics in the Rhizosphere.", Applied and Environmental Microbiology, 75(14), pp. 4790-4800. doi : 10.1128/AEM.02643-08
- Zahariev, M., Dahl, V., Chen, W., and Lévesque, C.A. (2009). "Efficient algorithms for the discovery of DNA oligonucleotide barcodes from sequence databases.", Molecular Ecology Resources, 9(Suppl. 1), pp. 58-64. doi : 10.1111/j.1755-0998.2009.02651.x
Other Science Related Publications:
2012:
- Barasubiye, T., Seifert, K.A., and Lévesque, C.A. (2012). "The National fungal identification service at ECORC_challenges and opportunities.", Canadian Phytopathological Society (CPS) 83rd Annual Meeting and International PPV Meeting, Marriott Gateway On The Falls, Niagara Falls, ON, Canada, June 24-27, 2012, Published in: Canadian Journal of Plant Pathology / Revue canadienne de phytopathologie, 35(1), pp. 102.
- Lévesque, C.A. (2012). "DNA Barcoding – Building Up and Using the Reference Database for fungi.", Canadian Wheat Improvement Workshop, National Research Council Plant Biotechnology Institute, Saskatoon, SK, Canada, March 21-22, 2012. (Presentation)
- Lévesque, C.A., Bilkhu, S., Chapados, J.T., Chen, W., Hambleton, S., Lewis, C.T., Oliver, K., and Seifert, K.A. (2012). "AeroNet: A Canadian spore trapping network monitoring fungi and bacteria with DNA barcodes and next generation sequencing.", The Role of Genomics & Bioinformatics in Protecting Canadians from Microbial Food Safety Risks Workshop, Southway Hotel and Conference Centre, Ottawa, ON, Canada, February 21-22, 2012. (Presentation)
- Lévesque, C.A., Robideau, G.P., Chen, W., and Lewis, C.T. (2012). "Oomycete barcoding and applications.", Inoculum, 63(3), pp. 28.
2011:
- Bala, K., Lévesque, C.A., and Saville, B. (2011). "Comparative analysis of gene expression in Pythium aphanidermatum during root-rot infection in multiple hosts.", Great Lakes - St. Lawrence Spring Workshop in Mycology, Peterborough, ON, Canada, May 7-8, 2011. (Presentation)
- Bala, K., Lévesque, C.A., and Saville, B. (2011). "Differential gene expression responses of Pythium aphanidermatum within plant hosts during root rot disease development.", Plant Canada 2011, Saint Mary’s University, Halifax, NS, Canada, July 17-21, 2011.
- Chen, W., Lewis, C.T., Chapados, J.T., Bilkhu, S., Hambleton, S., Seifert, K.A., and Lévesque, C.A. (2011). "Aerobiota diversity in Canada’s air: Mining the metagenomic data.", Great Lakes - St. Lawrence Spring Workshop in Mycology, Peterborough, ON, Canada, May 7-8, 2011. (Presentation)
- Duncan, R.W. and Lévesque, C.A. (2011). "Platforms supporting GRDI pilot projects.", GRDI Scientific Planning Meeting, NRC, Ottawa, ON, Canada, June 21, 2011. (Presentation)
- Eggertson, Q.A. and Lévesque, C.A. (2011). "Resolving the Pythium ultimum species complex.", Great Lakes - St. Lawrence Spring Workshop in Mycology, Peterborough, ON, Canada, May 7-8, 2011.
- Hambleton, S., Chen, W., Lewis, C.T., Bilkhu, S., Chapados, J.T., Oliver, K., Tenuta, A.U., and Lévesque, C.A. (2011). "AeroNet Canada: Profiling rust fungus diversity in spore trap samples with DNA barcoding and next generation sequencing.", North American Rust Workers Meeting (NARW), St. Paul, MN, USA, June 12, 2011. (Presentation)
- Lévesque, C.A., Bilkhu, S., Chapados, J.T., Chen, W., Hambleton, S., Lewis, C.T., Oliver, K., and Seifert, K.A. (2011). "AeroNet: A Canadian spore trapping network monitoring fungi and bacteria with DNA barcodes and next generation sequencing.", iBOL-Canada: The future of DNA Barcoding in Canada, Beaty Biodiversity Museum, UBC, Vancouver, BC, Canada, April 29-May 1, 2011. (Presentation)
- Lévesque, C.A., Robideau, G.P., Eggertson, Q.A., Rodrigue, N., Chen, W., Spies, C.F.J., Bala, K., de Cock, A.W.A.M., Hamilton, J., Adhikari, B., and Buell, C.R. (2011). "A case study with oomycetes on new approaches in taxonomy and ecology using next generation sequencing and comparative genomics.", The Research Horizons Seminar Series, McGill University, Macdonald Campus, Montréal, QC, Canada, October 27, 2011. (Presentation)
- Lévesque, C.A., Robideau, G.P., Eggertson, Q.A., Rodrigue, N., Chen, W., Spies, C.F.J., de Cock, A.W.A.M., Hamilton, J., Adhikari, B., and Buell, C.R. (2011). "Comparative genomics opens up a new era for oomycete taxonomy, ecology and diagnostics.", 71th Annual Meeting Meeting of the North-East Division of the American Phytopathological Society: The Oomycetes: Current and Future Challenges, New Brunswick, NJ, USA, October 12-14, 2011.
- Robideau, G.P. and Lévesque, C.A. (2011). "Flagellin-like filaments of Pythium aphanidermatum: function and phylogeny.", Great Lakes - St. Lawrence Spring Workshop in Mycology, Peterborough, ON, Canada, May 7-8, 2011.
- Robideau, G.P., Subramaniam, R., and Lévesque, C.A. (2011). "Inoculation of cucumber seedlings with purified Pythium flaggellar protein: Effect on seedling growth and response to subsequent attack by Pythium aphanidermatum zoospores.", 4th International Biofumigation & Biopesticides Symposium, Sheraton Cavalier Hotel, Saskatoon, SK, Canada, October 18-21, 2011, Book:15.
2010:
- Kang, S., Mansfield, M.A., Park, B., Geiser, D.M., Ivors, K.L., Coffey, M.D., Grünwald, N.J., Martin, F.N., Lévesque, C.A., and Blair, J.E. (2010). "The promise and pitfalls of sequence-based identification of plant-pathogenic fungi and oomycetes (Letter to Editor).", Phytopathology, 100(8), pp. 732-737.
2009:
- Lévesque, C.A. (2009). "Fungal species "expression" with oligoarrays and RT-PCR.", Canadian Journal of Plant Pathology / Revue canadienne de phytopathologie, 31(1), pp. 115. (Abstract)
- Lévesque, C.A., Seifert, K.A., Babcock, C., Bissett, J.D., Hambleton, S., Lewis, C.T., Redhead, S.A., Brière, S.C., Côté, M.J., and Hamelin, R.C. (2009). "CRTI-04-0045RD - Development of collections, reference/DNA databases and detection systems to counter bioterrorism against agriculture and forestry.", 7th Annual CRTI Summer Symposium 2009, Ottawa, ON, Canada, June 16-18, 2009, pp. 10-11.